Dr. Patrick Dolan Investigator and Unit Chief National Institutes of Health Host: Eric MayUsing Deep Mutational Scanning to Understand Enterovirus Capsid Function Viral capsid proteins are remarkable molecular machines. They have evolved to be robust to the environmental stresses encountered in the environment, which often includes changes in pH, salinity, and temperature, while maintaining precise mechanisms to release their genomic cargo when specific criteria are met - usually when receptors and co-receptors are presented within the right chemical environment. I'll present our work using mutational libraries encompassing all possible substitutions in the structural proteins of two prototype Enteroviruses, EV-D68 and EV-A71, to probe capsid functions involved in entry and uncoating. The results highlight specific regions that respond to chemical and biophysical changes, and identify functional sequences that may engage host factors to initiate and facilitate uncoating.About Dr. Dolan:Patrick T. Dolan, Ph.D., is an experimental virologist and computational biologist whose work focuses primarily on the evolution and host-virus interactions of positive-sense RNA viruses. In the fall of 2021, Patrick began as unit chief of the Quantitative Virology and Evolution Unit at NIAID in Bethesda, MD, where he studies the forces that shape the long- and short-term evolution of RNA virus populations. Patrick earned his B.S. degree in microbiology and molecular genetics from Michigan State University, where he worked in the laboratory of Professor Yong-Hui Zheng on the antiviral function of APOBEC3 cytidine deaminases in HIV-1. Patrick earned his Ph.D. in biological sciences in 2014 from Purdue University, where he studied the form and function of the hepatitis C virus-host protein interaction network under the supervision of professor Douglas J. LaCount and co-advisor professor Michael Gribskov. Patrick then pursued postdoctoral studies at Stanford University and University of California, San Francisco, in the laboratories of professors Raul Andino and Judith Frydman where he developed methods to understand the evolutionary dynamics of enteroviruses and flaviviruses in alternative host environments.Deep mutation, insertion and deletion scanning across the Enterovirus A proteome reveals constraints shaping viral evolution (https://pubmed.ncbi.nlm.nih.gov/39609576/)Structure and dynamics of enterovirus genotype networks (https://pubmed.ncbi.nlm.nih.gov/39609576/)Dolan Lab Website (https://qveu.github.io/QVEU/)

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

The Flex MBA Program offers a flexible, self-paced hybrid learning experience designed to fit your unique schedule and career goals. With a blend of online and in-person classes, students can customize their coursework to balance work, life, and education. In-person sessions are available at our Hartford and Stamford campuses, providing opportunities for networking and collaboration. The program is open to domestic and international students who require visa sponsorship.

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

For the full class schedule, descriptions, and to register, please visit the UConn Recreation website (https://recreation.uconn.edu/group-fitness-schedule/).

The exhibiting artists are Marsha Borden, Helena Chastel, Kathryn Frund, Phoebe Godfrey, Hugh MacDonald, Bob Pavlik, Dan Potter, and R. Douglass Rice. Open Air 2025 is open daily and will remain on view through October 6, 2025. June 19, 2025 iis the last day to visit indoor art exhibitions. Exhibitions inside the AVS Gallery will resume on September 11, 2025